Pistacia vera and its Combination with Cisplatin: A Potential Anticancer Candidate by Modulating Apoptotic Genes


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Introduction:Many bioactive phytochemicals have essential significance for handling various diseases and developing new drugs. The aim was to investigate the anti-tumor activity and the underlying mechanisms of pistachio pericarp extract (PPE) and pistachio kernel extract (PKE) alone and combined with cisplatin (CP) in the treatment of prostate cancer.

Methods:fThe effects of the PPE, PKE, and CP alone and PPE and PKE in combination with CP (PPE+CP and PKE+CP) on the proliferation of PC-3 cells were determined using the MTT assay. The fold changes of BAX, BCL-2, P53, KLK2, TNF, TGF, and NANOG expression against β-actin were determined by real-time technique. Data were analyzed by two-way ANOVA and repeated measure tests.

Results:These research results indicated that a greater anti-proliferative effect of the PPE and PKE was shown in combination with CP compared with treatments using the PPE and PKE or CP alone. The extracts and Cisplatin in vitro had good synergistic effects on the inhibition of the proliferation of PC-3 cells. The IC50 values of PKE+CP were 4.141, 2.140, and 0.884 ug/mL, and PPE+CP were 2.754, 2.061, and 0.753 ug/mL after 24 h, 48 h, and 72h treatment, respectively. Also, this result presented that the mRNA expression of BAX and P53 increased, and BCL-2, KLK2, TNF, TGF, and NANOG decreased in PC-3 cells.

Conclusions:The finding of this research showed for the first time the anti-carcinogenesis effects of separately and in the combination of PPE, PKE, and CP on the PC-3 prostate cancer cells via modulating some genes and that it may be nominated for the herbal anti-cancer medications.

Об авторах

Soudeh Khanamani Falahati-Pour

Pistachio Safety Research Center,, Rafsanjan University of Medical Sciences

Email: info@benthamscience.net

Seyedeh Torabizadeh

Department of Medicinal Chemistry, Kerman University of Medical Sciences

Email: info@benthamscience.net

Fatemeh Baghery

Pistachio Safety Research Center,, Rafsanjan University of Medical Sciences

Email: info@benthamscience.net

Mojgan Noroozi-Karimabad

Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran., Rafsanjan University of Medical Sciences

Автор, ответственный за переписку.
Email: info@benthamscience.net

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