Cell Death Induced by the Combination of Ephedra sinica Extract and Radiation in HNSCC is Positively Related to BAX and p-MLKL Expression

  • Авторы: Woo S.R.1, Noh J.K.2, Ahn S.3, Lee M.K.2, Yu H.S.4, Min S.4, Kong M.4, Lee J.W.5, Lee Y.C.6, Ko S.7, Eun Y.8
  • Учреждения:
    1. Department of Otolaryngology-Head and Neck Surgery, Kyung Hee University School of Medicine,, Kyung Hee University Medical Center
    2. Department of Biomedical Science and Technology, Graduate School,, Kyung Hee University
    3. Department of Otolaryngology-Head and Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center,
    4. Department of Radiation Oncology, Kyung Hee University School of Medicine, Kyung Hee University Medical Center
    5. Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyung Hee University,
    6. Department of Otolaryngology-Head and Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center
    7. Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University
    8. Department of Otolaryngology-Head and Neck Surgery, Kyung Hee University School of Medicine, , Kyung Hee University Medical Center
  • Выпуск: Том 24, № 1 (2024)
  • Страницы: 50-57
  • Раздел: Oncology
  • URL: https://snv63.ru/1871-5206/article/view/643675
  • DOI: https://doi.org/10.2174/0118715206267182231024105837
  • ID: 643675

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Аннотация

Background:Numerous studies have proven the efficacy and safety of natural products, and are widely used as attractive cancer treatments. The investigation of effective natural products for improving cancer treatment is a promising strategy. Combination treatment with radiosensitizers and radiotherapy (RT) is considered necessary for therapeutic improvement in head and neck squamous cell carcinoma(HNSCC).

Objective:This study aims to investigate whether Ephedra sinica (ES) extract could induce selective cell death in cancer cells and serve as a radiosensitizer for HNSCC.

Methods:HNSCC cells were pretreated with ES extract before radiation, and the radiosensitizing activity was assessed using a colony formation assay. Radiation-induced cell death was evaluated using an annexinV-FITC assay. Western blotting was performed to confirm cell death-related gene expression, including apoptosis and necrosis markers.

Results:ES extract significantly inhibited HNSCC cell viability (FaDu and SNU1076), while having minimal effect on normal HaCaT cells. When HNSCC cells were irradiated with 2, 4, or 8 Gy and cultured with ES extract (25 µg/mL), they exhibited increased radiation sensitivity compared to non-treated cells. The combination of ES extract and radiation resulted in increased cell death compared to non-treated, ES-treated, or irradiated cells. The apoptosis marker BAX and necrosis marker p-MLKL expression levels were also elevated following the combination treatment.

Conclusion:ES extract demonstrated significant cytotoxic potential in HNSCC cells without affecting normal cells. It enhanced the radiosensitivity of HNSCC cells by upregulating BAX and p-MLKL expression, leading to increased cell death. These results suggest ES extract exhibits a potential radiosensitizing capacity in HNSCC.

Об авторах

Seon Woo

Department of Otolaryngology-Head and Neck Surgery, Kyung Hee University School of Medicine,, Kyung Hee University Medical Center

Email: info@benthamscience.net

Joo Noh

Department of Biomedical Science and Technology, Graduate School,, Kyung Hee University

Email: info@benthamscience.net

Sun-Young Ahn

Department of Otolaryngology-Head and Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center,

Email: info@benthamscience.net

Min Lee

Department of Biomedical Science and Technology, Graduate School,, Kyung Hee University

Email: info@benthamscience.net

Hyeon Yu

Department of Radiation Oncology, Kyung Hee University School of Medicine, Kyung Hee University Medical Center

Email: info@benthamscience.net

Soonki Min

Department of Radiation Oncology, Kyung Hee University School of Medicine, Kyung Hee University Medical Center

Email: info@benthamscience.net

Moonkyoo Kong

Department of Radiation Oncology, Kyung Hee University School of Medicine, Kyung Hee University Medical Center

Email: info@benthamscience.net

Jung Lee

Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyung Hee University,

Email: info@benthamscience.net

Young Lee

Department of Otolaryngology-Head and Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center

Email: info@benthamscience.net

Seong-Gyu Ko

Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University

Email: info@benthamscience.net

Young-Gyu Eun

Department of Otolaryngology-Head and Neck Surgery, Kyung Hee University School of Medicine, , Kyung Hee University Medical Center

Автор, ответственный за переписку.
Email: info@benthamscience.net

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