Karanjin, A Promising Bioactive Compound Possessing Anti-cancer Activity against Experimental Model of Non-small Cell Lung Cancer Cells

  • 作者: Kumar G.1, Pandey D.M.2, Ghosh M.3, Dall'Acqua S.4, Gupta R.5, Tiwari N.2, Mohd Siddique U.6, Vishwakrama L.7, Guleri S.K.8, Lal U.9, Dubey S.10
  • 隶属关系:
    1. Department of Bioengineering and Biotechnology,, Birla Institute of Technology
    2. Department of Bio-Engineering and Biotechnology, Birla Institute of Technology, Mesra
    3. Department of Pharmaceutical Sciences and Technology,, Birla Institute of Technology, Mesra
    4. Department of Pharmaceutical Science and Pharmacology, University of Padua
    5. Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra
    6. Department of Pharmaceutical Sciences and Technology,, Birla Institute of Technology,
    7. Department of Microbiology, Government Medical College & Hospital
    8. Department of Community Medicine, Government Medical College & Hospital
    9. Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Birla Institute of Technology, Mesra
    10. Department of Chemistry, Kanya Gurukul Campus, Gurukul Kangri (Deemed to be University),
  • 期: 卷 24, 编号 5 (2024)
  • 页面: 317-333
  • 栏目: Oncology
  • URL: https://snv63.ru/1871-5206/article/view/644173
  • DOI: https://doi.org/10.2174/0118715206255557231024095245
  • ID: 644173

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Aims:The aim of this study is to isolate the Millettia pinnata (Karanj) leaf extract for pure compound with anticancer properties and to study the molecular target of the isolates in non-small cell lung cancer cell lines.

Background:In our earlier research Millettia pinnata leaf extract has demonstrated potential anticancer activities. Thus, in pursuit of the bioactive compounds, the most potential active extract from our previous study was purified. Furthermore, the anticancer properties of the isolated compound karanjin was studied and aimed for apoptosis and restraining growth

Methods:A novel method was developed through column chromatography for isolation and purification of the compound karanjin from leaf chloroform extract. The purified component was then characterised using FTIR, mass spectrometry, and NMR. An MTT-based cytotoxicity assay was used to analyse cell cytotoxicity, whereas fluorescence staining was used for apoptosis and reactive oxygen species inhibition quantification. Furthermore, the real-time PCR assay was used to determine the molecular mechanism of action in cells causing cytotoxicity induced by karanjin dosing

Results:The anticancer activity of karanjin in A549 cell line exhibited prominent activity revealing IC50 value of 4.85 µM. Conferring the predicted molecular pathway study, karanjin restrains the proliferation of cancer cells through apoptosis, which is controlled by extrinsic pathway proteins FAS/FADD/Caspases 8/3/9. Downregulation of KRAS and dependent gene expression also stopped cell proliferation.

Conclusion:Karanjin has been identified as a compound with potential effect in non-small cell lung cancer cells. Molecular mechanism for apoptosis and inhibition of reactive oxygen species induced through H2O2 were observed, concluding karanjin have medicinal and antioxidant properties.

作者简介

Gourav Kumar

Department of Bioengineering and Biotechnology,, Birla Institute of Technology

编辑信件的主要联系方式.
Email: info@benthamscience.net

Dev Pandey

Department of Bio-Engineering and Biotechnology, Birla Institute of Technology, Mesra

Email: info@benthamscience.net

Manik Ghosh

Department of Pharmaceutical Sciences and Technology,, Birla Institute of Technology, Mesra

Email: info@benthamscience.net

Stefano Dall'Acqua

Department of Pharmaceutical Science and Pharmacology, University of Padua

Email: info@benthamscience.net

Rashmi Gupta

Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra

Email: info@benthamscience.net

Nishi Tiwari

Department of Bio-Engineering and Biotechnology, Birla Institute of Technology, Mesra

Email: info@benthamscience.net

Usman Mohd Siddique

Department of Pharmaceutical Sciences and Technology,, Birla Institute of Technology,

Email: info@benthamscience.net

Leena Vishwakrama

Department of Microbiology, Government Medical College & Hospital

Email: info@benthamscience.net

Sunil Guleri

Department of Community Medicine, Government Medical College & Hospital

Email: info@benthamscience.net

Uma Lal

Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Birla Institute of Technology, Mesra

Email: info@benthamscience.net

Supriya Dubey

Department of Chemistry, Kanya Gurukul Campus, Gurukul Kangri (Deemed to be University),

Email: info@benthamscience.net

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