Apoptosis-inducing Metabolite from Marine Mangrove Actinobacteria VITGAP173


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Аннотация

Background:Marine actinobacteria have proven to be a remarkable source of bioactive metabolites.

Methods:The present study focused on the isolation of anticancer metabolites from marine actinobacteria. Streptomyces sp. VITGAP173 was found to have promising anticancer activity against breast cancer cell lines (MCF-7).

Results:Bioassay-guided fractionation was followed to identify the bioactive metabolites from crude ethyl acetate extract of VITGAP173, which yielded four fractions. Fraction B exhibited the highest cytotoxic activity against MCF-7 cell lines among the four fractions. Further structural characterization of the fraction was done by FTIR and NMR spectroscopy. The fraction-2 induced cytotoxicity against MCF-7 cell lines and the half maximal inhibition (IC50) value was calculated as 4.7 µg/ml. To elucidate the possible mechanism of cell death, MCF-7 cells were treated with fraction-2 for 24 hours and the morphological changes were examined using acridine orange – ethidium bromide (AO/EB) staining. The fraction also increased the reactive oxygen species (ROS) generation (Flow cytometry, DCFHDA). The molecular mechanism of fraction-induced cell death was analysed by real-time PCR, which revealed that the fraction promotes apoptosis through the CHOP-ATF-4 pathway involved in ER stress signalling.

Conclusion:The present findings suggested the apoptosis-inducing potential of fraction-2 in breast cancer therapy.

Об авторах

Ajitha Gomathi

Department of Biotechnology, School of BioSciences and Technology,, Vellore Institute of Technology (VIT)

Email: info@benthamscience.net

Manikandan Alagumuthu

, M.S. Ramaiah College of Arts, Science and Commerce,

Email: info@benthamscience.net

Pavan Sai

Department of Biotechnology, School of BioSciences and Technology, Vellore Institute of Technology (VIT)

Email: info@benthamscience.net

Harishkumar Madhyastha

Department of Applied Physiology, Faculty of Medicine, University of Miyazaki

Email: info@benthamscience.net

Rama Jayaraj

, Northern Territory Institute of Research and Training

Email: info@benthamscience.net

Gothandam Muthukailannan

Department of Biotechnology, School of BioSciences and Technology, Vellore Institute of Technology (VIT)

Автор, ответственный за переписку.
Email: info@benthamscience.net

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