Ginsenoside Rb1 Inhibits the Proliferation of Lung Cancer Cells by Inducing the Mitochondrial-mediated Apoptosis Pathway


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Background:Lung cancer is one of the more common malignant tumors posing a great threat to human life, and it is very urgent to find safe and effective therapeutic drugs. The antitumor effect of ginsenosides has been reported to be a treatment with a strong effect and a high safety profile.

Objective:This paper aimed to investigate the inhibitory effect of ginsenoside Rb1 on 95D and NCI-H460 lung cancer cells and its pathway to promote apoptosis.

Methods:We performed the CCK-8 assay, fluorescence staining assay, flow cytometry, scratch healing assay, and Transwell assay to detect the effects of different concentrations of ginsenoside Rb1 on the antitumor activity of 95D and NCI-H460 cells and Western Blot detected the mechanism of antitumor effect.

Results:Ginsenoside Rb1 treatment significantly increased the inhibition and apoptosis rates of 95D and NCIH460 cells and inhibited the cell cycle transition from S phase to G2/M. Rb1 induces apoptosis by altering the levels of P53, Bax, Cyto-c, Caspase-8, Caspase-3, Cleaved Caspase-3, Bcl-2, MMP-2, and MMP-9 proteins and activating the external apoptotic pathway.

Conclusion:Ginsenoside Rb1 inhibits proliferation and migration and induces apoptosis of 95D and NCI-H460 lung cancer cells by regulating the mitochondrial apoptotic pathway to achieve antitumor activity.

Sobre autores

Lin Feng

College of Pharmacy, Changchun University of Chinese Medicine

Email: info@benthamscience.net

Xinze Liu

Jilin Ginseng Academy, Changchun University of Chinese Medicine

Email: info@benthamscience.net

Kaijing Sun

Jilin Ginseng Academy, Changchun University of Chinese Medicine

Email: info@benthamscience.net

Ying Sun

, The Second Hospital of Dalian Medical University

Email: info@benthamscience.net

Wei Wu

Jilin Ginseng Academy, Changchun University of Chinese Medicine

Email: info@benthamscience.net

Changbao Chen

Jilin Ginseng Academy, Changchun University of Chinese Medicine

Email: info@benthamscience.net

Xin Jin

Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University

Autor responsável pela correspondência
Email: info@benthamscience.net

Xilin Wan

Jilin Ginseng Academy, Changchun University of Chinese Medicine

Autor responsável pela correspondência
Email: info@benthamscience.net

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