Mesenchymal Stem Cell-derived Type II Alveolar Epithelial Progenitor Cells Attenuate LPS-induced Acute Lung Injury and Reduce P63 Expression


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Abstract

Aim:Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a severe clinical respiratory-failure disease mainly characterized by acute damage to the alveolar epithelium and pulmonary vascular endothelial cells. Stem cell therapy has emerged as a potential regenerative strategy for ARDS/ALI, however, the outcome is limited, and the underlying mechanisms are unclear.

Introduction:We established a differentiation system for bone marrow-derived mesenchymal stem cellderived (BM-MSC) type II alveolar epithelial progenitor cells (AECIIs) and assessed their regulatory effects on lipopolysaccharide (LPS)-induced ALI.

Methods:We induced BM-MSC differentiation into AECIIs using a specific conditioned medium. After 26 days of differentiation, 3×105 BM-MSC-AECIIs were used to treat mice with LPS-induced ALI through tracheal injection.

Results:After tracheal injection, BM-MSC-AECIIs migrated to the perialveolar area and reduced LPSinduced lung inflammation and pathological injury. RNA-seq suggested that P63 protein was involved in the effects of BM-MSC-AECIIs on lung inflammation.

Conclusion:Our results suggest that BM-MSC-AECIIs may reduce LPS-induced acute lung injury by decreasing P63 expression.

About the authors

Ning Ma

Inflammation & Allergic Diseases Research Unit, The Affiliated Hospital of Southwest Medical University

Email: info@benthamscience.net

Mengwei Zhang

Inflammation & Allergic Diseases Research Unit, The Affiliated Hospital of Southwest Medical University

Email: info@benthamscience.net

Guofeng Xu

Inflammation & Allergic Diseases Research Uni, The Affiliated Hospital of Southwest Medical University

Email: info@benthamscience.net

Lifang Zhang

Department of Neurosurgery, The Affiliated Hospital of Southwest Medical University

Email: info@benthamscience.net

Min Luo

Inflammation & Allergic Diseases Research Unit, The Affiliated Hospital of Southwest Medical University

Email: info@benthamscience.net

Meihua Luo

Inflammation & Allergic Diseases Research Unit, The Affiliated Hospital of Southwest Medical University

Email: info@benthamscience.net

Xing Wang

Inflammation & Allergic Diseases Research Unit, The Affiliated Hospital of Southwest Medical University

Email: info@benthamscience.net

Hongmei Tang

Inflammation & Allergic Diseases Research Unit, The Affiliated Hospital of Southwest Medical University

Email: info@benthamscience.net

Xiaoyun Wang

Inflammation & Allergic Diseases Research Unit, The Affiliated Hospital of Southwest Medical University

Email: info@benthamscience.net

Li Liu

Laboratory of Anesthesiology, Southwest Medical University

Email: info@benthamscience.net

Xiaolin Zhong

Department of Gastroenterology, The Affiliated Hospital of Southwest Medical University

Email: info@benthamscience.net

Jianguo Feng

Laboratory of Anesthesiology, Southwest Medical University

Email: info@benthamscience.net

Yuying Li

Inflammation & Allergic Diseases Research Unit, The Affiliated Hospital of Southwest Medical University

Author for correspondence.
Email: info@benthamscience.net

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