Mesenchymal Stem Cell-conditioned Medium Protecting Renal Tubular Epithelial Cells by Inhibiting Hypoxia-inducible Factor-1α and Nuclear Receptor Coactivator-1
- Авторы: Liao C.1, Liu Y.1, Lin Y.1, Wang J.1, Zhou T.1, Weng W.1
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Учреждения:
- Department of Nephrology, Second Affiliated Hospital of Shantou University Medical College
- Выпуск: Том 19, № 10 (2024)
- Страницы: 1369-1381
- Раздел: Medicine
- URL: https://snv63.ru/1574-888X/article/view/645475
- DOI: https://doi.org/10.2174/011574888X247652230928064627
- ID: 645475
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Аннотация
Background:Acute kidney injury (AKI) is characterized by inflammatory infiltration and damage and death of renal tubular epithelial cells (RTECs), in which hypoxia plays an important role. Deferoxamine (DFO) is a well-accepted chemical hypoxia-mimetic agent. Mesenchymal stem cell-conditioned medium (MSC-CM) can reduce local inflammation and repair tissue. In this study, we explored the effect and molecular mechanism of MSC-CM-mediated protection of RTECs under DFO-induced hypoxia.
Methods:Rat renal proximal tubule NRK-52E cells were treated with different concentrations of DFO for 24 hours, followed by evaluation of RTEC injury, using a Cell Counting Kit-8 (CCK-8) to detect cell viability and western blotting to evaluate the expression of transforming growth factor- beta 1 (TGF-β1), α-smooth muscle actin (α-SMA), and hypoxia-inducible factor-1 alpha (HIF-1α) in NRK-52E cells. Then, three groups of NRK-52E cells were used in experiments, including normal control (NC), 25 µM DFO, and 25 µM DFO + MSC-CM. MSC-CM was obtained from the human umbilical cord. MSC-CM was used to culture cells for 12 hours before DFO treatment, then fresh MSC-CM and 25 µM DFO were added, and cells were cultured for another 24 hours before analysis.
Results:Western blotting and cellular immunofluorescence staining showed culture of NRK-52E cells in 25 µM DFO for 24 hours induced HIF-1α and nuclear receptor coactivator-1 (NCoA-1), simulating hypoxia. MSC-CM could inhibit the DFO-induced up-regulation of α-SMA, TGF-β1, HIF-1α and NCoA-1.
Conclusion:Our results suggest that MSC-CM has a protective effect on RTECs by down-regulating HIF-1α and NCoA-1, which may be the harmful factors in renal injury.
Об авторах
Chunling Liao
Department of Nephrology, Second Affiliated Hospital of Shantou University Medical College
Email: info@benthamscience.net
Yiping Liu
Department of Nephrology, Second Affiliated Hospital of Shantou University Medical College
Email: info@benthamscience.net
Yongda Lin
Department of Nephrology, Second Affiliated Hospital of Shantou University Medical College
Email: info@benthamscience.net
Jiali Wang
Department of Nephrology, Second Affiliated Hospital of Shantou University Medical College
Email: info@benthamscience.net
Tianbiao Zhou
Department of Nephrology, Second Affiliated Hospital of Shantou University Medical College
Автор, ответственный за переписку.
Email: info@benthamscience.net
Wenjuan Weng
Department of Nephrology, Second Affiliated Hospital of Shantou University Medical College
Автор, ответственный за переписку.
Email: info@benthamscience.net
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