Underlying Mechanism of Traditional Herbal Formula Chuang-Ling-Ye in the Treatment of Diabetic Foot Ulcer through Network Pharmacology and Molecular Docking
- Authors: Geng J.1, Zhou G.2, Guo S.3, Ma C.4, Ma J.1
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Affiliations:
- State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University
- Department of General Surgery, Affiliated Hospital of Nanjing University of Chinese Medicine
- Department of General Surgery,, Affiliated Hospital of Nanjing University of Chinese Medicine
- Department of General Surgery,, Nanjing University of Chinese Medicine
- Issue: Vol 30, No 6 (2024)
- Pages: 448-467
- Section: Immunology, Inflammation & Allergy
- URL: https://snv63.ru/1381-6128/article/view/646003
- DOI: https://doi.org/10.2174/0113816128287155240122121553
- ID: 646003
Cite item
Full Text
Abstract
Background:Chuang-Ling-Ye (CLY) has been clinically proven to be an effective Chinese medicine for the treatment of diabetic foot ulcers (DFU).
Objectives:This study aimed to investigate the possible mechanism of CLY in relation to DFU using network pharmacology and molecular docking.
Materials and Methods:Firstly, relevant targets of CLY against DFU were obtained from TCMSP, Swiss Target Prediction database and GEO database. Then, topological analysis was employed by Cytoscape to screen the top 6 core active ingredients and the top 8 hub targets. Furthermore, the OmicShare Tools were applied for gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis. Finally, the results of network pharmacology were verified by molecular docking method.
Results:CLY has 61 active compounds and 361 targets after de-duplication, and the top 8 hub targets were EGFR, TP53, CCND1, IL-1B, CREBBP, AR, PTGS2 and PGR. GO enrichment analysis is mainly related to signal transducer activity, receptor activity, and molecular transducer activity. KEGG pathway analysis indicated that these shared targets were primarily focused on AGE-RAGE signaling pathway in diabetic complications, HIF-1 signaling pathway, IL-17 signaling pathway, and JAK-STAT signaling pathway. Molecular docking results showed that physciondiglucoside, 2-cinnamoyl-glucose and kinobeon A were well bound with EGFR, IL-1B, AR and PTGS2.
Conclusion:This study demonstrated that CLY has anti-oxidative stress and anti-inflammatory effects in the treatment of DFU through various constituents, multiple targets, and multiple pathways, which provides a valuable point of reference for future investigations on CLY.
About the authors
Jinyuan Geng
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University
Email: info@benthamscience.net
Guowei Zhou
Department of General Surgery, Affiliated Hospital of Nanjing University of Chinese Medicine
Email: info@benthamscience.net
Song Guo
Department of General Surgery,, Affiliated Hospital of Nanjing University of Chinese Medicine
Email: info@benthamscience.net
Chaoqun Ma
Department of General Surgery,, Nanjing University of Chinese Medicine
Email: info@benthamscience.net
Jiangfeng Ma
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University
Author for correspondence.
Email: info@benthamscience.net
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Supplementary files
