Neuro-protective Effect of Acetyl-11-keto-β-boswellic Acid in a Rat Model of Scopolamine-induced Cholinergic Dysfunction


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Abstract

Background::Acetyl-11-keto-β-boswellic acid (AKBA) is a major component of the oleo-gum resin of B. serrata with multiple pharmacological activities. The objective of this study was to explore the underlying mechanisms of neuroprotective potential of AKBA against scopolamine-mediated cholinergic dysfunction and memory deficits in rats.

Methods::The rats received AKBA (2.5, 5, and 10 mg/kg, oral) for 21 days. In the third week, scopolamine was administered 30 min before the Morris water maze and passive avoidance tests. In order to perform biochemical assessments, the hippocampus and prefrontal cortex were extracted from the rats euthanized under deep anesthesia.

Results::In the MWM test, treatment with AKBA (5 and 10 mg/kg) decreased the latency and distance to find the platform. Moreover, in the PA test, AKBA remarkably increased latency to darkness and stayed time in lightness while decreasing the frequency of entry and time in the darkness. According to the biochemical assessments, AKBA decreased acetylcholinesterase activity and malondialdehyde levels while increasing antioxidant enzymes and total thiol content. Furthermore, AKBA administration restored the hippocampal mRNA and protein levels of brain-derived neurotrophic factor (BDNF) and mRNA expression of B-cell lymphoma (Bcl)- 2 and Bcl-2- associated X genes in brain tissue of scopolamine-injured rats.

Conclusion::The results suggested the effectiveness of AKBA in preventing learning and memory dysfunction induced by scopolamine. Accordingly, these protective effects might be produced by modulating BDNF, cholinergic system function, oxidative stress, and apoptotic markers.

About the authors

Amir Hossein Assaran

Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences

Email: info@benthamscience.net

Mahmoud Hosseini

Department of Physiology, Mashhad University of Medical Sciences

Email: info@benthamscience.net

Matin Shirazinia

Neuroscience Research Center, Mashhad University of Medical Sciences

Email: info@benthamscience.net

Mohammad Hosein Eshaghi Ghalibaf

Applied Biomedical Research Center, Mashhad University of Medical Sciences

Email: info@benthamscience.net

Farimah Beheshti

Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences

Email: info@benthamscience.net

Leila Mobasheri

Pharmacology, Mashhad University of Medical Sciences

Email: info@benthamscience.net

Farshad Mirzavi

Cardiovascular Diseases Research Center, Birjand University of Medical Sciences

Email: info@benthamscience.net

Arezoo Rajabian

Neuroscience Research Center, Mashhad University of Medical Sciences

Author for correspondence.
Email: info@benthamscience.net

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