Membrane chromatographic test system for the determination of bisphenol A in drinking water based on the use of an aptamer

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A membrane test system has been developed for the rapid determination of bisphenol A in drinking water, utilizing a conjugate of gold nanoparticles with an aptamer that specifically binds the target analyte, and a conjugate of mercaptosuccinic acid with a carrier protein impregnated in the test zone of the strip. The working principle of the test system is based on the binding of free gold nanoparticles in the test zone, which are formed as a result of the competitive interaction of the aptamer with bisphenol A and its release from the surface of the gold nanoparticles. Conjugates of gold nanoparticles with aptamers of different compositions were obtained and tested. Optimal conditions were selected to achieve a low detection limit for bisphenol A. The developed test system allows for the detection of bisphenol A within 15 minutes with a detection limit of 13.5 ng/mL. The suitability of the test system was confirmed by testing drinking water; the detection rate of bisphenol A ranged from 88.2 to 101.3%.

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Sobre autores

N. Komova

Research Center of Biotechnology of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru

A. N. Bach Institute of Biochemistry

Rússia, 119071 Moscow

K. Serebrennikova

Research Center of Biotechnology of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru

A. N. Bach Institute of Biochemistry

Rússia, 119071 Moscow

A. Berlina

Research Center of Biotechnology of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru

A. N. Bach Institute of Biochemistry

Rússia, 119071 Moscow

A. Zherdev

Research Center of Biotechnology of the Russian Academy of Sciences

Email: dzantiev@inbi.ras.ru

A. N. Bach Institute of Biochemistry

Rússia, 119071 Moscow

B. Dzantiev

Research Center of Biotechnology of the Russian Academy of Sciences

Autor responsável pela correspondência
Email: dzantiev@inbi.ras.ru

A. N. Bach Institute of Biochemistry

Rússia, 119071 Moscow

Bibliografia

  1. Tarafdar A., Sirohi R., Balakumaran P.A., Reshmy R., Madhavan A., Sindhu R., Binod P., Kumar Y., Kumar D., Sim S.J. The hazardous threat of bisphenol A: Toxicity, detection and remediation // J. Hazard. Mater. 2022. V. 423. Article 127097.
  2. Ni L., Zhong J., Chi H., Lin N., Liu Z. Recent advances in sources, migration, public health, and surveillance of bisphenol A and its structural analogs in canned foods // Foods. 2023. V. 12. Article 1989.
  3. Mishra A., Goel D., Shankar S. Bisphenol A conta- mination in aquatic environments: A review of sources, environmental concerns, and microbial remediation // Environ. Monit. Assess. 2023. V. 195. Article 1352.
  4. Wang X., Nag R., Brunton N.P., Siddique M.A.B., Harrison S.M., Monahan F.J., Cummins E. Human health risk assessment of bisphenol A (BPA) through meat products // Environ. Res. 2022. V. 213. Article 113734.
  5. Abraham A., Chakraborty P. A review on sources and health impacts of bisphenol A // Rev. Environ. Health 2020. V. 35. P. 201.
  6. Ганичев П., Маркова О., Еремин Г., Зарицкая Е., Петрова М. Влияние бисфенола А на здоровье населения. Краткий литературный обзор // Здоровье – основа человеческого потенциала: проблемы и пути их решения. 2020. T. 15. C. 239.
  7. World Health Organization. Guidelines for Drinking Water Quality 3rd Ed. V. 1. Recommendations. 2008.
  8. Dang A., Sieng M., Pesek J.J., Matyska M.T. Determination of Bisphenol A in receipts and carbon paper by HPLC-UV // J. Liq. Chromatogr. Relat. Technol. 2015. V. 38. P. 438.
  9. Hadjmohammadi M.R., Saeidi I. Determination of bisphenol A in Iranian packaged milk by solid-phase extraction and HPLC // Monatsh. Chem. – Chemical Monthly. 2010. V. 141. P. 501.
  10. Alnaimat A.S., Barciela-Alonso M.C., Bermejo-Barrera P. Determination of bisphenol A in tea samples by solid phase extraction and liquid chromatography coupled to mass spectrometry // Microchem. J. 2019. V. 147. P. 598.
  11. Deceuninck Y., Bichon E., Marchand P., Boquien C.-Y., Legrand A., Boscher C., Antignac J.P., Le Bizec B. Determination of bisphenol A and related substitutes/analogues in human breast milk using gas chromatography-tandem mass spectrometry // Anal. Bioanal. Chem. 2015. V. 407. P. 2485.
  12. Cunha S.C., Inácio T., Almada M., Ferreira R., Fernandes J.O. Gas chromatography–mass spectrometry analysis of nine bisphenols in canned meat products and human risk estimation // Food Res. Int. 2020. V. 135. Article 109293.
  13. Mei Z., Chu H., Chen W., Xue F., Liu J., Xu H., Zhang R., Zheng L. Ultrasensitive one-step rapid visual detection of bisphenol A in water samples by label-free aptasensor // Biosens. Bioelectron. 2013. V. 39. P. 26.
  14. Zhang D., Yang J., Ye J., Xu L., Xu H., Zhan S., Xia B., Wang L. Colorimetric detection of bisphenol A based on unmodified aptamer and cationic polymer aggregated gold nanoparticles // Anal. Biochem. 2016. V. 499. P. 51.
  15. Lei Y., Zhang Q., Fang L., Akash M.S.H., Rehman K., Liu Z., Shi W., Chen S. Development and comparison of two competitive ELISAs for estimation of cotinine in human exposed to environmental tobacco smoke // Drug Test. Anal. 2014. V. 6. P. 1020.
  16. Bahadır E.B., Sezgintürk M.K. Lateral flow assays: Principles, designs and labels // TrAC, Trends Anal. Chem. 2016. V. 82. P. 286.
  17. Chatterjee S., Mukhopadhyay S. Recent advances of lateral flow immunoassay components as “point of need” // J. Immunoassay Immunochem. 2022. V. 43. P. 579.
  18. Mei Z., Deng Y., Chu H., Xue F., Zhong Y., Wu J., Yang H., Wang Z., Zheng L., Chen W. Immunochromatographic lateral flow strip for on-site detection of bisphenol A // Microchim. Acta. 2013. V. 180. P. 279.
  19. Mei Z., Qu W., Deng Y., Chu H., Cao J., Xue F., Zheng L., El-Nezamic H.S., Wu Y., Chen W. One-step signal amplified lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A (BPA) in aqueous samples // Biosens. Bioelectron. 2013. V. 49. P. 457.
  20. Chen A., Yang S. Replacing antibodies with aptamers in lateral flow immunoassay // Biosens. Bioelectron. 2015. V. 71. P. 230.
  21. Chen Z., Wu Q., Chen J., Ni X., Dai J. A DNA Aptamer based method for detection of SARS-CoV-2 nucleocapsid protein // Virologica Sinica. 2020. V. 35. P. 351.
  22. Dong H., Liu X., Gan L., Fan D., Sun X., Zhang Z., Wu P. Nucleic acid aptamer-based biosensors and their application in thrombin analysis // Bioanalysis. 2023. V. 15. P. 513.
  23. Yu H., Jing W., Cheng X. CRISPR-Cas- and aptamer-based systems for diagnosing pathogens: A Review // Zoonoses. 2023. V. 3. Artilce 22.
  24. Zhang W., Liu Q.X., Guo Z.H., Lin J.S. Practical application of aptamer-based biosensors in detection of low molecular weight pollutants in water sources // Molecules. 2018. V. 23. P. 344.
  25. Alkhamis O., Canoura J., Yu H., Liu Y., Xiao Y. Innovative engineering and sensing strategies for aptamer-based small-molecule detection. // TrAC, Trends Anal. Chem. 2019. V. 121. Article 115699.
  26. Adachi T., Nakamura Y. Aptamers: A review of their chemical properties and modifications for therapeutic application // Molecules. 2019. V. 24. P. 4229.
  27. Caglayan M.O., Şahin S., Üstündağ Z. An overview of aptamer-based sensor platforms for the detection of bisphenol-A // Crit. Rev. Anal. Chem. 2022. P. 1320.
  28. Rajabnejad S.-H., Badibostan H., Verdian A., Karimi G.R., Fooladi E., Feizy J. Aptasensors as promi-sing new tools in bisphenol A detection – An invisible pollution in food and environment // Microchem. J. 2020. V. 155. Article 104722.
  29. Shayesteh O.H., Ghavami R. Two colorimetric ampicillin sensing schemes based on the interaction of aptamers with gold nanoparticles // Microchim. Acta. 2019. V. 186. P. 1.
  30. Luo C., Wen W., Lin F., Zhang X., Gu H., Wang S. Simplified aptamer-based colorimetric method using unmodified gold nanoparticles for the detection of carcinoma embryonic antigen // RSC Adv. 2015. V. 5. P. 10994.
  31. Retnakumari A., Setua S., Menon D., Ravindran P., Muhammed H., Pradeep T., Nair S., Koyakutty M. Molecular-receptor-specific, non-toxic, near-infrared-emitting Au cluster-protein nanoconjugates for targeted cancer imaging // Nanotechnology. 2010. V. 21. Article 055103.
  32. Arivarasan A., Bharathi S., Ezhilarasi S., Arunpandiyan S., Jayavel R. Photovoltaic performances of Yb doped CdTe QDs sensitized TiO2 photoanodes for solar cell applications // J. Inorg. Organomet. Polym. Mater. 2019. V. 29. P. 859.
  33. Komova N.S., Serebrennikova K.V., Berlina A.N., Zherdev A.V., Dzantiev B.B. Membrane analytical test system for highly sensitive determination of Hg2+ ions in natural waters // Limnol. Freshw. Biol. 2022. V. 2022. P. 1305.
  34. Askari E., Naghib S.M. A novel approach to facile synthesis and biosensing of the protein-regulated graphene // Int. J. Electrochem. Sci. 2018. V. 13. P. 886.
  35. Giorgi-Coll S., Marín M.J., Sule O., Hutchinson P.J., Carpenter K.L.H. Aptamer-modified gold nanoparticles for rapid aggregation-based detection of inflammation: An optical assay for interleukin-6 // Microchim. Acta. 2019. V. 187. P. 13.
  36. Królikowska A., Bukowska J. Self-assembled monolayers of mercaptosuccinic acid on silver and gold surfaces designed for protein binding. Part I: Structure of the monolayer // J. Raman Spectrosc. 2007. V. 38. P. 936.

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2. Fig. 1. Schematic diagram of a membrane chromatographic test system using an aptamer for the determination of bisphenol A.

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3. Fig. 2. (a) IR spectra of bovine serum albumin (BSA-MYA conjugate) (1), mercaptosuccinic acid (MYA) (2) and bovine serum albumin (BSA) (3); (b) absorption spectrum of BSA-MYA conjugate.

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4. Fig. 3. Absorption spectra of (a) GNP-1 and (b) GNP-2 before (solid line) and after (dashed line) conjugation with aptamer. Electron microscopic images of (c) GNP-1-Apt and (d) GNP-2-Apt.

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5. Fig. 4. Hydrodynamic diameters of (a) GNP-1 and (b) GNP-2 and their conjugates with aptamer before and after reaction with bisphenol A.

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6. Fig. 5. Dependence of the intensity of test zone staining on the concentration of the bovine serum albumin-mercaptosuccinic acid conjugate.

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7. Fig. 6. Dependences of the intensity of test zone staining on the incubation time with bisphenol A (30 μg/ml), obtained using the conjugates GNP-1-Apt (dotted line) and GNP-2-Apt (solid line).

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8. Fig. 7. Dependences of the test zone staining intensity on the concentration of bisphenol A for (a) GNP-1-Apt and (b) GNP-2-Apt. Insert: digital images of test strips at different concentrations of bisphenol A. Experimental conditions: bovine serum albumin−mercaptosuccinic acid 3 mg/ml, 100 μl of methanol−water mixture (1:4), 2 μl of GNP-Apt solution, incubation time 10 min.

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9. Fig. 8. Selectivity of bisphenol A determination in comparison with other substances that have a negative effect on the reproductive system – intensity of test zone coloration when testing solutions with a concentration of 1 μg/ml. The dotted line indicates the limit of naked eye observation of test line coloration.

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