Determination of isoniazid by photometric method through covalent binding with carbocyanine dye

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Resumo

Substitution reactions in carbocyanine dyes are used to determine nucleophilic compounds. The interaction of a chlorine-containing carbocyanine with a number of medicinal substances has been studied. It was shown that in the reaction with isoniazid in the presence of surfactants, this dye selectively changes color from yellow-green to purple. Chromatography-mass spectrometry has proven the formation of the substitution product of chlorine with isoniazid. The reaction occurs within 20 minutes in the presence of 1 mM cetyltrimethylammonium bromide. The detection limit of isoniazid in water by photometric method was 10 µg/mL; in diluted artificial urine using fluorimetry, it was 0.3 µg/mL. The method does not require full-spectrum equipment, which simplifies the determination.

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Sobre autores

E. Skorobogatov

M. V. Lomonosov Moscow State University

Autor responsável pela correspondência
Email: skoregy@gmail.com

Department of Chemistry

Rússia, 119991 Moscow

Yu. Timchenko

M. V. Lomonosov Moscow State University

Email: skoregy@gmail.com

Department of Chemistry

Rússia, 119991 Moscow

I. Doroshenko

M. V. Lomonosov Moscow State University

Email: skoregy@gmail.com

Department of Chemistry

Rússia, 119991 Moscow

T. Podrugina

M. V. Lomonosov Moscow State University

Email: skoregy@gmail.com

Department of Chemistry

Rússia, 119991 Moscow

I. Rodin

M. V. Lomonosov Moscow State University

Email: skoregy@gmail.com

Department of Chemistry

Rússia, 119991 Moscow

M. Beklemishev

M. V. Lomonosov Moscow State University

Email: skoregy@gmail.com
Rússia, 119991 Moscow

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1. JATS XML
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2. Scheme 1. Structural formulas of dyes; (a) – dye 1, (b) – IR-783.

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3. Scheme 2. Substitution of a chlorine atom in a dye.

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4. Fig. 1. (a) – Intensity of the green channel (G) in photographs of a plate with the dye-CTAB system in the presence of various drugs (1 mM), reaction duration 20 min; (b) – example of a photograph of a fluorimetric plate, the cell of the system with isoniazid is marked in red.

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5. Fig. 2. (a) – Absorption spectra of the dye-isoniazid mixture at the initial time (1) and 20 min after the start of the reaction (3); the dye-CTAB-isoniazid mixture at the initial time (2), after 20 min (4) and 90 min (5); (b) – near-IR fluorescence spectra of the dye-CTAB-isoniazid-artificial urine system at the initial time in the presence of 1 mM isoniazid (1) and after 20 min (5); and the dye-CTAB-isoniazid-artificial urine system at the initial time in the presence of 0.1 mM isoniazid at the initial time (2), after 20 min (3) and 90 min (4).

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6. Fig. 3. Dependence of the green channel intensity (G) for the dye–CTAB and dye–CTAB–isoniazid systems on pH (1 – in the absence of isoniazid, 2 – in the presence of 1 mM isoniazid). Reaction duration is 20 min.

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7. Fig. 4. Dependence of the intensity of the green channel (G) of the dye–CTAB–isoniazid system on the concentration of CTAB for 1 mM isoniazid: 1 – in the absence of isoniazid, 2 – in the presence of 1 mM isoniazid; control samples (without CTAB) are marked in black. The reaction duration is 20 min.

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8. Fig. 5. Effect of medicinal substances (1 mM) in dye–CTAB (blue columns) and dye–CTAB-isoniazid (1 mM isoniazid, red columns) systems on the intensity of the green channel. Control – systems without interfering compounds. Reaction duration is 20 min.

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9. Fig. 6. Dependence of fluorescence intensity in the near IR region of the spectrum for dye–CTAB and dye–CTAB–isoniazid systems on pH: 1 – in the absence of isoniazid, 2 – in the presence of 1 mM isoniazid. Reaction duration is 20 min.

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