Assembly of a Complex of Immature 30S Ribosomal Subunit and Era GTPase Maturation Factor from Staphylococcus aureus in vitro for Structural Studies

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Abstract

Staphylococcus aureus Era GTPase is one of the ribosome maturation factors. Era GTPase is involved in the late stages of small (30S) ribosome subunit assembly and regulates formation of the entire 70S ribosome. Study of the structure of the Era and 30S subunit complex from S. aureus will help in studying the processes of ribosome maturation and mechanisms of protein synthesis regulation in this pathogenic microorganism. This paper presents protocols for obtaining Era GTPase and immature 30S ribosome subunits from S. aureus, as well as a protocol for assembling their complex for further structural studies.

About the authors

E. A. Klochkova

Kazan Federal University

Email: konstantin.usachev@kpfu.ru
Kazan, 420008 Russia

A. D. Biktimirov

Kazan Federal University, Kazan, 420008 Russia

Kazan, 420008 Russia

A. G. Bikmullin

Kazan Federal University

Kazan, 420008 Russia

N. S. Garaeva

Kazan Federal University

Kazan, 420008 Russia

M. M. Yusupov

National Research Center “Kurchatov Institute”; Institute of Genetics, Molecular and Cellular Biology, CNRS UMR7104, INSERM U964, Universit’e de Strasbourg

Moscow, 123182 Russia; Illkirch, F‑67400 France

K. S. Usachev

Kazan Federal University

Kazan, 420008 Russia

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