Variability of the promoter-operator region of Bacillus cereus hlyii gene impacts on its transcriptional activity level

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Abstract

The promoter-operator region of the Bacillus cereus hlyII gene includes an elongated operator region with mirror symmetry, recognizable with the main specific transcriptional regulator HlyIIR. In addition, regions for the global transcription regulators Fur, OhrR, and ResD, are located in the region of the hlyII gene operator. The latter is a transcriptional regulator of the redox-sensitive ResDE signal transduction pathway. Bacillus cereus sensu lato strains were found with a disturbance in the proximal part of the area recognized by HlyIIR and ResD. The essential role of these regions in the expression of the hlyII gene has been demonstrated. Natural strains of Bacillus cereus with deletions in the proximal region of the HlyIIR operator of the hlyII gene have a significantly reduced expression level of hlyII were identified. Disturbances in HlyIIR operator reduce the expression of hlyII several tens of times. The presence of an intact recognition site for ResD reduces the expression of this gene several times under aerobic conditions. These results allow us to determine the influence of structural variability in the promoter-operator region of Bacillus cereus hlyII genes on its transcriptional activity.

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About the authors

A. M. Shadrin

FSBIS FRC Pushchino Scientific Centre of Biological Research, Russian Academy of Sciences

Email: solonin.a.s@yandex.ru

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Russian Federation, Moscow oblast, Pushchino, 142290

E. V. Shapyrina

FSBIS FRC Pushchino Scientific Centre of Biological Research, Russian Academy of Sciences

Email: solonin.a.s@yandex.ru

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Russian Federation, Moscow oblast, Pushchino, 142290

A. S. Nagel

FSBIS FRC Pushchino Scientific Centre of Biological Research, Russian Academy of Sciences

Email: solonin.a.s@yandex.ru

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Russian Federation, Moscow oblast, Pushchino, 142290

A. V. Siunov

FSBIS FRC Pushchino Scientific Centre of Biological Research, Russian Academy of Sciences

Email: solonin.a.s@yandex.ru

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Russian Federation, Moscow oblast, Pushchino, 142290

Zh. I. Andreeva-Kovalevskaya

FSBIS FRC Pushchino Scientific Centre of Biological Research, Russian Academy of Sciences

Email: solonin.a.s@yandex.ru

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Russian Federation, Moscow oblast, Pushchino, 142290

V. I. Salyamov

FSBIS FRC Pushchino Scientific Centre of Biological Research, Russian Academy of Sciences

Email: solonin.a.s@yandex.ru

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Russian Federation, Moscow oblast, Pushchino, 142290

A. S. Solonin

FSBIS FRC Pushchino Scientific Centre of Biological Research, Russian Academy of Sciences

Author for correspondence.
Email: solonin.a.s@yandex.ru

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Russian Federation, Moscow oblast, Pushchino, 142290

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2. Fig. 1. Comparison of the amino acid sequences of ResD and the binding sites of Bacillus subtilis and Bacillus cereus. a – the result of alignment of the amino acid sequences of ResD using the ClustalΩ program. b – comparison of the promoter-operator regions of hlyII in Bacillus cereus strains B-771 with a disruption of the ResD operator and B-370 with a deletion of the HlyIIR operator region from –196 to –228 nucleotides.

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