Influence of ABA-metabolizing bacteria on the growth and aba content in wheat plants and soil in dense planting

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Abstract

The influence of abscisic acid (ABA)-metabolizing bacterial strains of the genera Pseudomonas and Arthrobacter on the hormone content in the shoots and roots of wheat plants, as well as in a sandy substrate during dense planting, has been studied. The ability of bacteria to reduce the ABA content in the growing environments and in plants, albeit to varying degrees, was discovered already 10 days after inoculation. At the same time, most of the studied strains were characterized by decrease of ABA in shoots, and suppression of ABA in the roots was observed less frequently. The simultaneous decrease in the hormone in the shoots and in the sandy substrate, which manifested itself under the influence of strains P. plecoglossicida 2.4-D, P. frederiksbergensis IB Ta10m, P. veronii IB K11-1, was accompanied by a maximum increase of wheat weight plants as compared to non-inoculated plants. The studied bacteria to varying degrees stimulated the accumulation of shoot and root mass, as well as leaf area, which could probably be associated with their different ability to synthesize other hormones, such as IAA, and/or influence the hormonal system of the plant itself. The prospects of using ABA-destructor bacteria for the development of agricultural biological products that can mitigate the negative effects of thickened crops and increase resistance to other abiotic factors are discussed.

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About the authors

E. V. Martynenko

Ufa Institute of Biology–Ufa Federal Research Center of the RAS

Email: vysotskaya@anrb.ru
Russian Federation, prosp. Oktyabrya 71, Ufa 450054

L. Y Kuzmina

Ufa Institute of Biology–Ufa Federal Research Center of the RAS

Email: vysotskaya@anrb.ru
Russian Federation, prosp. Oktyabrya 71, Ufa 450054

E. R. Gaffarova

Ufa Institute of Biology–Ufa Federal Research Center of the RAS

Email: vysotskaya@anrb.ru
Russian Federation, prosp. Oktyabrya 71, Ufa 450054

А. S. Ryabova

Ufa Institute of Biology–Ufa Federal Research Center of the RAS

Email: vysotskaya@anrb.ru
Russian Federation, prosp. Oktyabrya 71, Ufa 450054

G. R. Kudoyarova

Ufa Institute of Biology–Ufa Federal Research Center of the RAS

Email: vysotskaya@anrb.ru
Russian Federation, prosp. Oktyabrya 71, Ufa 450054

L. B. Vysotskaya

Ufa Institute of Biology–Ufa Federal Research Center of the RAS

Author for correspondence.
Email: vysotskaya@anrb.ru
Russian Federation, prosp. Oktyabrya 71, Ufa 450054

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2. Fig. 1. The mass of the whole plant, shoot and root, the ratio of the root/shoot masses of wheat plants 13 days after planting 5, 10 and 20 seedlings in one vegetative vessel. Different letters indicate significantly different values ​​(n = 30, P ≤0.05, ANOVA, Duncan's test). The same in Figs. 2–5.

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3. Fig. 2. Nitrogen balance index NBI (a) and chlorophyll content (b) of wheat plants 13 days after planting 5, 10 and 20 seedlings in one vegetation vessel.

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4. Fig. 3. ABA content in shoots and roots (ng/g raw weight) of wheat plants and in soil (ng/vessel) 10 days after planting the seedlings in a vessel and introducing ABA destructors into the rhizosphere (up to 108 CFU/g substrate) (n = 9).

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5. Fig. 4. The mass of shoots (a) and roots (b) 10 days after planting wheat seedlings in vessels and introducing ABA destructors into the rhizosphere (up to 108 CFU/g of substrate) (n = 12).

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6. Fig. 5. Total leaf area of ​​wheat plants 10 days after planting and introduction of ABA destructors into the rhizosphere (up to 108 CFU/g of substrate) (n = 12).

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