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Species diversity and photobiont localization specificities in epigene lichens (on the example of the genus Cladonia)

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1. Title Title of document Species diversity and photobiont localization specificities in epigene lichens (on the example of the genus Cladonia)
2. Creator Author's name, affiliation, country Evgene Sergeevich Korchikov; Samara National Research University; Russian Federation
2. Creator Author's name, affiliation, country Egor Vladimirovich Bolgov; Samara National Research University; Russian Federation
2. Creator Author's name, affiliation, country Elena Sergeevna Ilyina; Samara National Research University; Russian Federation
2. Creator Author's name, affiliation, country Timofey Anatolyevich Pankratov; Lomonosov Moscow State University; Russian Federation
3. Subject Discipline(s)
3. Subject Keyword(s) symbiotic organisms; photobiont; Asterochloris; Stichococcus; Chlorella; Pseudococcomyxa; microbial communities of lichens; taxonomic structure of symbiosis; dynamics of algal abundance; north-taiga zone; steppe zone; BBS MSU; Murmansk Region; Orenburg Region
4. Description Abstract

The article describes the taxonomic composition and photobionts localization specificities in above-ground lichens of the genus Cladonia. Moreover it analyzes the amount and algal species in thallus homogenates due to their age: in zones of active growth, aging zone and destruction zone; alga cells in washings from surface of thalli. Also the article analyzes the influence of the mycobiont on the photobiont species and abundance of the photobiont. It gives the comparison analysis of these data in lichens in the north taiga zone (Murmansk Region) and steppe zone (on the example of Orenburg Region). The method of direct microscopy helped to determine the taxonomic affiliation of the photobionts to the genus. Here the authors mean the size, shape of the cells, the nature of the chromatophore, etc. The number of photobionts in samples was studied both by light microscopy and fluorescence microscopy. The paper presents the comparison results of the amount and algae diversity in in the thalli of the above-ground lichens of the genus Cladonia due to their geographical location and the specificity of the age dynamics of the podecium. The research shows the fact that Asterochloris alga was the dominant group in the actively growing lichen podecia. However geographical location is not important. Chlorella, Stichococcus, Pseudococcomyxa were found in lichens algae. The authors believe that the new information about the changes in the taxonomic composition of algae, due to the age of the thallus is important for science. Pseudococcomyxa algae dominated in the old parts of the north taiga lichens and Stichococcus dominated in the steppe areas. Moreover the authors give the attempt of resynthesize lichen tallium from previously isolated cultures of the mycobiotic and photobiont. This attempt was unsuccessful at the period of the research work.

5. Publisher Organizing agency, location Samara State University of Social Sciences and Education
6. Contributor Sponsor(s) This work was supported by the Russian Foundation for Basic Research (project No. 16-04-00966a).
7. Date (DD-MM-YYYY) 15.08.2018
8. Type Status & genre Peer-reviewed Article
8. Type Type Research Article
9. Format File format
10. Identifier Uniform Resource Identifier https://snv63.ru/2309-4370/article/view/21688
10. Identifier Digital Object Identifier (DOI) 10.17816/snv201873111
10. Identifier Digital Object Identifier (DOI) (PDF (Rus)) 10.17816/snv201873111.t17928
11. Source Title; vol., no. (year) Samara Journal of Science; Vol 7, No 3 (2018)
12. Language English=en ru
13. Relation Supp. Files Figure 1 - Micrographs of cultures of photobionts from the lichen Cladonia arbuscula, growing in the Orenburg region: A - Asterochloris sp .; B - Chlorella sp .; B - Stichococcus sp. Magnification × 280 (A), × 400 (B, C) (46KB) doi: 10.17816/snv201873111.f46409
Figure 2 - The number of algal cells in different functional zones of the epigeic lichen Cladonia arbuscula growing in different natural zones (30KB) doi: 10.17816/snv201873111.f46410
Figure 3 - Dynamics of changes in the number of photobionts of the genus Asterochloris in lichens C. arbuscula (CA) of the Orenburg region (OR), Khibiny (KH) and the Biological station of Moscow State University (Murmansk region) (B); C. rangiferina (CR) and C. stellaris (CS): 1 - apex, 2 - middle, 3 - basal part of subecia (27KB) doi: 10.17816/snv201873111.f46411
Figure 4 - Scales of the mycobiont Cladonia alpestris: A - Asterochloris sp. on scales; B - branching of scales resembling subdetia (× 14) (29KB) doi: 10.17816/snv201873111.f46412
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
15. Rights Copyright and permissions Copyright (c) 2018 Korchikov E.S., Bolgov E.V., Ilyina E.S., Pankratov T.A.
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