Samara Journal of ScienceSamara Journal of Science2309-43702782-3016Samara State University of Social Sciences and Education2167810.17816/snv201873101Research ArticleFuture outlook of plant growth regulators application influencing callus induction from different type explant <i>Hyoscyamus muticus</i> L. <i>in vitro</i>AbdelazeezWalla Abdelmaksood<p>postgraduate student of Botany and Plant Physiology Department</p>wallamohamed68@gmail.comKhusnetdinovaLandysh Zavdetovna<p>candidate of biological sciences, associate professor of Botany and Plant Physiology Department</p>husnetdinova.l@mail.ruTimofeevaOlga Arnoldovna<p>doctor of biological sciences, professor of Botany and Plant Physiology Department</p>olga.timofeeva@kpfu.ruKazan (Volga Region) Federal University1508201873101310032020Copyright © 2018, Abdelazeez W.A., Khusnetdinova L.Z., Timofeeva O.A.2018<p>The article shows the results concerning the problem of the influence of the hormonal composition of the medium on callus induction in isolated from different explants of Egyptian henbane areas (on the example of <em>Hyoscyamus muticus</em> L.). The authors study 11 variants of Murashige and Skoog medium supplemented with different concentrations and combination of auxins and cytokinins. It was important to find nutrient medium modification of Murashige and Skoog for callus induction. The article describes the fact that callus formation from different explant types of <em>Hyoscyamus muticus</em> L. <em>in vitro</em> was observed on Murashige and Skoog medium fortified with benzylaminopurine and naphthylacetic acid. It shows that the maximum callus induction was observed from root explants on Murashige and Skoog's medium supplemented with 0.5 mg/l of benzylaminopurine and 1.0 mg/l of naphthylacetic acid. And minimal callus formation was observed in the area with benzylaminopurine. Callus induction of leaf and stem explants both on the hormone-free nutrient medium and with the benzylaminopurine only was not observed. Thus, the results show that the frequency of callus formation with culturing root segment is higher compared to leaf and stem segment explants (on the example of Egyptian henbane in culture <em>in vitro</em>). This work aims to inducing callus formation from various explants of Egyptian henbane, which can be used for plant regeneration or as a source for <em>in vitro</em> production of secondary metabolites.</p>Hyoscyamus muticus L.egyptian henbancallus formationin vitro cultureexplantsecondary metabolitesauxincytokininbenzylaminopurinenaphthylacetic acidsegments of leaf, stem, rootMurashige and Skoog mediumHyoscyamus muticus L.белена египетскаякаллусообразованиекультура in vitroэксплантвторичные метаболитыауксинцитокининбензиламинопуриннафтилуксусная кислотасегменты листа, стебля, корнясреда Мурасиге и Скуга[Khater M.A., Elashtokhy M.M.A. Effect of growth regulators on in vitro production of Hyoscyamus aureus L. and tropane alkaloids // International journal of ChemTech Research. 2015. Vol. 8 (11). P. 113-119.][Basu P. Tropane alkaloids from callus cultures, differentiated roots and shoots of Hyoscyamus muticus L. // Plant Biochem Biotechnol. 1998. 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